The extracellular portion of the LAG-3 protein corresponds to amino acids 1 to 420 of the LAG-3 protein described above.
Comparing the sequence of the LAG-3 gene represented by c DNA FDC above as well as the organization exon / intron of the LAG-3 gene with other Ig / SF-like molecules revealed a close relationship the LAG-3 protein with the CD4 protein.
The existence of this protein naturally on T cells has been demonstrated by: - preparation of sera raised against a synthetic peptide representing a region probably exposed to the outside of the FDC c DNA translation product having a protein structure looped, - immunoprecipitation of the LAG-3 protein by anti-peptide heteroantibodies.
The proteins of the invention can further be obtained by other methods of purification of membrane proteins or conventional peptide synthesis or by application of genetic engineering techniques comprising inserting a DNA sequence encoding a protein according to the invention in an expression vector such as a plasmid and transformation of cells with this expression vector and culturing these cells.
The present invention therefore relates to a DNA sequence comprising the nucleotide sequence designated FDC, corresponding to the c DNA sequence shown in SEQ ID No. The translation begins at nucleotide 231 and ends at nucleotide 1724 inclusive.
The present invention also relates to the protein encoded by FDC, namely LAG-3 protein shown in Sequence ID No. The first 28 amino acids should form a signal sequence that is removed in the mature protein.
The present invention thus more particularly relates to the protein corresponding to the protein sequence 1 to 470 of SEQ ID No. The mature protein is a membrane protein of type I of 470 amino acids, the theoretical molecular weight deduced from the protein structure is 51295 Daltons and point isoélec stick equal to 10.9.
For this, some of the amino acids of the encoded protein are sometimes replaced without changing the activity.
Ho aggiunto dell'erba cipollina fresca surgelata e del coriandolo fresco anche surgelato e cotto per circa 20 minuti a pentola coperta.
Proteins produced by human lymphocytes are described, particularly a protein which is expressed on their surface.
Other applications of these genetic engineering techniques based on the same principles allow experimental with prokaryotic or eukaryotic expression systems to produce virtually unlimited quantities protein whose gene was discovered.
The inventors have sought to discover new genes encoding membrane proteins not described to date.